graduate student
Russian Federation
employee
Russian Federation
employee
Russian Federation
employee
Russian Federation
V stat'e rassmatrivaetsya vliyanie somaticheskogo ekstrakta Anisakis simplex L3 na kul'tury kletok mikroorganizmov in vitro. Ranee ustanovleno, chto pod deystviem ukazannogo ekstrakta narushaetsya i ugnetaetsya process deleniya eukarioticheskih kletok. Svedeniy o mehanizmah vzaimodeystviya somaticheskih ekstraktov gel'mintov i mikroorganizmov ochen' malo. Predpolagaetsya, chto somaticheskiy ekstrakt iz anizakid okazyvaet negativnoe vliyanie na mikroorganizmy za schet vhodyaschih v ego sostav belkovyh komponentov i metabolitov. Cel'yu issledovaniya yavlyalos' izuchenie vliyaniya ekstrakta na kul'tury kletok raznoobraznyh mikroorganizmov, kak po morfologicheskim priznakam, tak i po ustoychivosti k faktoram vneshney sredy. Ekstrakt gotovili iz lichinok anizakid, izvlechennyh iz zamorozhennoy putassu (Micromesistius poutassou), proveryali na steril'nost' i bezvrednost', opredelyali soderzhanie belka. Dlya issledovaniya ispol'zovali sutochnye kul'tury bakteriy: mikrokokki Micrococcus sp., palochki Escherichia coli, Proteus vulgaris, Salmonella tiphimurium i bacilly Bacillus subtilis. Pri kul'tivirovanii mikroorganizmov s diskami, propitannymi antigennym ekstraktom anizakid, v termostate pri +37 °S cherez 12 chasov vyyavlena zona zaderzhki rosta u Micrococcus sp., E. coli i P. vulgaris. Na rost bakteriy palochek S. tiphimurium, bacill B. subtilis ekstrakt vliyaniya ne okazyval. Formirovanie vyrazhennoy zony steril'nosti svidetel'stvuet o nalichii v sostave belkovogo ekstrakta biologicheski-aktivnyh komponentov, obladayuschih bakteriostaticheskim deystviem. Obsuzhdayutsya mehanizmy bakteriostaticheskogo deystviya somaticheskogo ekstrakta. Poluchennye v hode eksperimenta dannye podtverzhdayut antagonisticheskie otnosheniya belkovyh produktov lichinok Anisakis simplex L3 i mikroorganizmov, soglasuyuschihsya s literaturnymi dannymi. Vyyavlena razlichnaya stepen' aktivnosti antigenov somaticheskogo ekstrakta iz anizakid na kul'tury mikroorganizmov, naibol'shee vliyanie ekstrakt okazal na kul'tury palochek E. coli i mikrokokkov Micrococcus sp. Poluchennye rezul'taty mozhno ispol'zovat' dlya razrabotki modeli bakterial'nyh kletok kak test ob'ektov dlya ocenki antigennoy aktivnosti somaticheskih, ekskretorno-sekretornyh ekstraktov i metabolitov razlichnyh gel'mintov.
somaticheskiy ekstrakt, Anisakis simplex L3, metabolity, bakterii, bacilly, bakteriostaticheskoe deystvie.
1. MUK 4.2.1890-04. Opredelenie chuvstvitel'nosti mikroorganizmov k antibakterial'nym preparatam : metodicheskie ukazaniya. M. : Federal'nyy centr Gossanepidnadzora Minzdrava Rossii, 2004. 125 c.
2. Volkova L. V., Grishina T. A., Volkov A. G. Nizkomolekulyarnye kationnye peptidy leykocitov, inducirovannye razlichnymi antigenami // Vestnik PNIPU. Himicheskaya tehnologiya i biotehnologiya. 2015. № 4. S. 35-48.
3. Sivkova T. N. Poluchenie i harakteristika antigenov gel'mintov : uchebno-metodicheskoe posobie / sost. T. N. Sivkova. Perm' : Permskaya GSHA, 2009. 14 s.
4. Sivkova T. N. Kariopaticheskoe i patomorfologicheskoe deystvie produktov metabolizma lichinok anizakid : monografiya. Perm' : Permskaya GSHA, 2011. 132 s.
5. Abner S. R., Parthasarathy G., Hill D. E., Mansfield L. S. Trichuris suis: detection of antibacterial activity in excretory-secretory products from adults // Exp. Parasitol. 2001. No. 99. R. 26-36.
6. Andersson M., Boman A., Boman H. H. Ascaris nematodes from pig and human make three antibacterial peptides: isolation of cecropin P1 and two ASABF peptides // Cell. Mol. Life Sci. 2003. No. 60. R. 599-606.
7. Audicana M. T., Kennedy M. W. Anisakis simplex: from obscure infectious worm to inducer of immune hypersensitivity // Clin Microbiol. Rev. 2008. No. 21. R. 360-379.
8. Belas R., Manos J., Suvanasuthi R. Proteus mirabilis ZapA metalloprotease degrades a broad spectrum of substrates, including antimicrobial peptides // Infect. Immun. 2004. No. 72. R. 5159-5167.
9. Delmar J. A., Su C. C., Yu E. W. Bacterial multidrug efflux transporters // Annu. Rev. Biophys. 2014. No. 43. R. 93-117.
10. Drake L., Korchev Y., Bashford L., Djamgoz M., Wakelin D., Ashall F. et al. The major secreted product of the whipworm, Trichuris, is a pore-forming protein // Proc. Biol. Sci. 1994. No. 257. R. 255-261.
11. Eberle R., Brattig N.W., Trusch M., Schlüter H., Achukwi M. D., Eisenbarth A., Renz A., Liebau E., Perbandt M., Betzel C. Isolation, identification and functional profile of excretory-secretory peptides from Onchocerca ochengi // Acta Trop. 2015. No. 142. R. 156-166.
12. Fæste C. K., Jonscher K. R., Dooper M. et al. Characterisation of potential novel allergens in the fish parasite Anisakis simplex // EuPA Open Proteomics. 2014. No. 4. R. 140-155.
13. Fæste C. K. Fish feed as source of potentially allergenic peptides from the fish parasite Anisakis simplex (S. L.) // Animal feed science and technology. 2015. No. 202. R. 52-61.
14. Haarder S., Kania P. W., Holm T. L., Gersdorff J. L., Buchmann K. Effect of ES products from Anisakis (Nematoda: Anisakidae) on experimentally induced colitis in adult zebra fish // Parasite Immunol. 2017. V. 39. I. 10. P. 66.
15. Joo H. S., Fu C. I., Otto M. Bacterial strategies of resistance to antimicrobial peptides // Philos Trans. R. Soc. Lond. B. Biol. Sci. 2016. No. 26. P. 1-11.
16. Kato Y. Humoral defense of the nematode Ascaris suum: antibacterial, bacteriolytic and agglutinating activities in the body fluid // Zoolog. Sci. 1995. No. 12. R. 225-230.
17. Mehrdana F., Buchmann K. Excretory secretory products of anisakid nematodes: biological and pathological roles // Acta Veterinaria Scandinavica. 2017. No. 59:42. R. 1-12.
18. Midha A., Schlosser J., Hartmann S. Reciprocal Interactions between Nematodes and Their Microbial Environments // Frontiers in Cellular and Infection Microbiology. 2017. V. 7:144. R. 1-20.
19. Peschel A. et al. Staphylococcus aureus resistance to human defensins and evasion of neutrophil killing via the novel virulence factor Mpr F is based on modification of membrane lipids with L-lysine // J. Exp. Med. 2001. No. 193. R. 1067-1076.
20. Reynolds L. A., Finlay B. B., Maizels R. M. Cohabitation in the intestine: interactions between helminth parasites, bacterial microbiota and host immunity // Journal of immunology. 2015. 195. No. 9. R. 4059-4066.
21. Schmidtchen A., Frick I. M., Andersson E., Tapper H., Bjorck L. Proteinases of common pathogenic bacteria degrade and inactivate the antibacterial peptide LL-37 // Mol. Microbiol. 2002. No. 46. R. 157-168.
22. Silhavy T. J., Kahne D., Walker S. The bacterial cell envelope. Cold Spring Harb. // Perspect. Biol. 2010. No. 2 (5). P. 87.
23. Shelton C. L., Raffel F. K., Beatty W. L., Johnson S. M., Mason K. M. Sap transporter mediated import and subsequent degradation of antimicrobial peptides in Haemophilus // PLoS Pathog. 2011. No. 7 (11). P. 44.
24. Svanevik C. S., Lunestad B. T., Levsen A. Effect of Anisakis simplex (sl) larvae on the spoilage rate and shelflife of fish mince products under laboratory conditions // Food Control. 2014. No. 46. R. 121-126.
25. Tarr D. E. K. Distribution and characteristics of ABFs, cecropins, nemapores, and lysozymes in nematodes // Dev. Comp. Immunol. 2012. No. 36. R. 502-520.
26. Wardlaw A. C., Forsyth L. M., Crompton D.W. Bactericidal activity in the pig roundworm Ascaris suum // J. Appl. Bacteriol. 1994. No. 76. R. 36-41.
27. Zhang H., Yoshida S., Aizawa T., Murakami R., Suzuki M., Koganezawa N. et al. In vitro antimicrobial properties of recombinant ASABF, an antimicrobial peptide isolated from the nematode Ascaris suum // Antimicrob. Agents Chemother. 2000. No. 44. R. 2701-2705.
