Abstract. The area of natural saline lands in Russia is about 30 million hectares. At present, the growth of technogenically saline areas is associated with the extraction of minerals (agro-ore) and the formation of drill pits when drilling wells for the purpose of exploration and production of hydrocarbons. The number of drilling pits with a high salt content in the Khanty-Mansiysk autonomous okrug is about 3 thousand, about the same number of them in the Yamal-Nenets autonomous okrug, in the south of the Tyumen region their number is increasing in the Uvat region. Reclamation of these territories includes technical and biological stages of reclamation. The biological stage is associated with the use of phytomeliorant cultures. The inclusion of a legume component in the composition of phytomeliorants assumes its joint use with the preparation of an active strain of nodule bacteria. The aim of the research is to identify the most salt-tolerant strain of lupine nodule bacteria at different chemistry and degree of salinity of the environment. Research methodology and methods. Laboratory experiments in Petri dishes were used to test lupine nodule bacteria for salt resistance and salinity chemistry. Nodule bacteria of lupine strains 363a, 367a, 375a were taken directly from rhizotorphin preparations, the degree of dilution was brought to a certain titer corresponding to about 80 colonies per Petri dish. Nodule bacteria for melilot were represented by strains 282, 292 (CL) and 291, and for alfalfa, respectively, 451b, 404b, 412b, 423b, 408b and 425a. They were seeded with the same titer. Results. The presented results of experiments showed that colonies of lupine nodule bacteria react extremely negatively to the salt concentration in the nutrient medium of 0.3 % or higher. This is typical for both neutral and soda salinity. Of the three studied strains of nodule bacteria, sample 367a was the most salt-resistant. Of the presented strains of sweet clover and alfalfa, strains 282 and 423b had the highest salt tolerance, respectively. Scientific novelty. For the first time, strains of lupine nodule bacteria have been tested for salt tolerance in relation to different chemistry and degree of salinity identical to technogenic soils and saline soils.
nodule bacteria, colonies, chemistry and degree of salinity, osmotic pressure, strains, pH of the medium
1. Alferov A. A., Chernova L. S., Kozhemyakov A. P. Efficacy of biopreparations for spring wheat in the european part of Russia against different backgrounds of mineral nutrition // Russian Agricultural Sciences. 2018. T. 44. No. 1. Pp. 53-57.
2. Ezhov G. I. Rukovodstvo k prakticheskim zadaniyam po sel'skohozyaystvennoy mikrobiologii. Moskva: Vysshaya shkola,1981. 271 s.
3. Vorobiev N. I., Pukhalsky Ya. V., Loskutov S., Sviridova O. V., Pishchik V. N., Osipov A. I., Yakubovskaya A. I., Kozhemyakov A. P. Agrotechnology for snapshots soil health with bacteria // In: International Scientific and Practical Conference “AgroSMART - Smart Solutions for Agriculture”. Tyumen, 2019. Pp. 488-496.
4. Kozhemyakov A. P. Perspektivy effektivnogo primeneniya zemleudobritel'nyh biopreparatov v zemledelii i rastenievodstve // Perspektivy ispol'zovaniya innovacionnyh form udobreniy, sredstv zaschity i regulyatorov rosta rasteniy v agrotehnologiyah sel'skohozyaystvennyh kul'tur: materialy dokladov uchastnikov 9-y nauchno-prakticheskoy konferencii «Anapa-2016». Anapa, 2016. S. 81-83.
5. Kozhemyakov A. P., Laktionov Yu. V., Popova T. A., Orlova A. G., Kokorina A. L., Vayshlya O. B., Agafonov E. V., Guzhvin S. A., Churakov A. A., Yakovleva M. T. Agrotehnologicheskie osnovy sozdaniya usovershenstvovannyh form mikrobnyh biopreparatov dlya zemledeliya // Sel'skohozyaystvennaya biologiya. 2015. T. 50. № 3. S. 369-376.
6. Laktionov Yu. V., Yahno V. V., Kozhemyakov A. P. Novye podhody v kul'tivirovanii i primenenii mikrobiologicheskih preparatov dlya rastenievodstva // Sovremennoe sostoyanie, problemy i perspektivy razvitiya agrarnoy nauk: materialy III Mezhdunarodnoy nauchnoy konferencii. 2018. S. 38-39.
7. Laktionov Yu. V., Kosul'nikov Yu. V., Dudnikova D. V., Yahno V. V., Kozhemyakov A. P. Ocenka ustoychivosti shtammov kluben'kovyh bakteriy soi k rekomenduemym himicheskim fungicidam // Zernovoe hozyaystvo Rossii. 2019. № 1 (61). S. 62-67.
8. Muntyan V. S., Akinina Yu. N., Simarov B. V., Rumyanceva M. L. Analiz strukturnogo polimorfizma gena puta, vovlechennogo v metabolizm prolina u prirodnyh shtammov Sinorhizobium meliloti // Aktual'naya biotehnologiya. 2018. № 3 (26). S. 429-431.
9. Petuhova V. S., Skipin L. N., Bogdanova O. G. Sovershenstvovanie sposobov rekul'tivacii burovyh shlamov. Tyumen': TIU, 2017. 140 s.
10. Posypanov G. S. Metody izucheniya biologicheskoy fiksacii azota vozduha. Moskva: Agropromizdat, 1991. 299 s.
11. Rumyanceva M. L., Vladimirova M. E., Muntyan V. S., Stepanova G. V., Saksaganskaya A. S., Kozhemyakov A. P., Orlova A. G., Becker A., Simarov B. V. Vysokoeffektivnye shtammy kluben'kovyh bakteriy lyucerny (Medicago Varia L.): molekulyarno-geneticheskaya harakteristika i ispol'zovanie v sopryazhennoy selekcii // Sel'skohozyaystvennaya biologiya. 2019. T. 54. № 6. S. 1306-1323.
12. Roumiantseva M. L., Muntyan V. S., Cherkasova M. E., Saksaganskaya A. S., Andronov E. E., Simarov B. V. Genomic islands in sinorhizobium meliloti RM1021, nitrogen-fixing symbiont of alfalfa // Russian Journal of Genetics. 2018. T. 54. No. 7. Pp. 759-769.
13. Saksaganskaya A. S., Muntyan V. S., Rumyanceva M. L. Polimorfizm genov kluben'kovyh bakteriy Sinorhizobium meliloti, determiniruyuschih virulentnost' i stressoustoychivost' // V knige: Biologiya - Nauka HHI veka. Sbornik tezisov. Magnitogorsk: KT «Buki-Vedi», 2016. S. 44-45.
14. Saksaganskaya A. S., Kozlova A. P., Muntyan V. S., Rumyanceva M. L. Dinamika obrazovaniya kluben'kov na kornyah lyucerny shtammami Sinorhizobium meliloti raznyh NOD-genotipov // Eksperimental'nye i teoreticheskie issledovaniya v sovremennoy nauke: sbornik statey po materialam V mezhdunarodnoy nauchno-prakticheskoy konferencii. Novosibirsk, 2017. S. 50-56.
15. Skipin L. N., Petuhova V. S., Perfil'ev N. V., Hramcov N. V. Parametry zhiznedeyatel'nosti kluben'kovyh bakteriy pri izmenenii edaficheskih faktorov // Vestnik KrasGAU. 2014. № 6 (93). S. 103-108.
16. Skipin L. N. Soloncy Sibiri: ekologicheskie aspekty osvoeniya. Tyumen', 2000. 261 s.
17. Fung T. M. V., Manucharova N. A., Stepanov A. L., Pozdnyakov L. A., Selickaya O. V., Emcev V. T. Agrobacterium tumefaciens - associativnaya azotfiksiruyuschaya bakteriya // Vestnik Moskovskogo universiteta. Seriya 17: Pochvovedenie. 2015. № 3. S. 50-55.
18. Yakobi L. M., Zheleznyakov S. V., Smetanin R. V., Lebedeva V. K., Kozhemyakov A. P. Issledovanie inducirovannogo mutanta lyucerny hmelevidnoy (Medicagolupulinal. SUBSP. Vulgariskoch) po simbioticheskim priznakam pri obrazovanii mikoriznogo simbioza // VII S'ezd Vavilovskogo obschestva genetikov i selekcionerov, posvyaschennyy 100-letiyu kafedry genetiki SPbGU, i associirovannye simpoziumy: sbornik tezisov Mezhdunarodnogo Kongressa. Sankt-Peterburg, 2019. S. 960.
19. Yakobi L. M., Zheleznyakov S. V., Kozhemyakov A.P. Morphological description of non-effective arbuscular mycorrhiza developed by plant mutant (Medicago lupulina L. Subsp. Vulgaris koch.) in assosiation with rhizophagus irregularis // PLAMIC 2018 “Plants and microorganisms: Biotechnology of the future”: proceedings of the international scientific conference. Ufa, 2018. P. 35.
