Abstract. Research objective was to study the intestinal microbiocenosis in broiler chickens. Laboratory test was conducted at Research and Development Center for Agriculture of the North-Western Branch of Tyumen Research Center of SB RAS. Methods. Cobb 500 chickens breed was used. The main part of experiment began at the age of 14 days. By this time, chickens had reached an average body weight of 365 grams. The control group of chickens was fed basic diet, consisting of total mixed ration fodder and pure water. Chickens of the 1st experimental group during the main stage of experiment were fed with “Vetom 2” probiotic additive in drinking water at 50 mg / 1 kg of poultry weight, whereas chickens of the 2nd experimental group were fed “Lactobifadol” added to their fodder at the rate of 0.2 g per 1 kg of poultry weight. Research results have showed that prior to feeding probiotic formulations no pathogenic microflora was observed in the microbiocenosis of the droppings of broiler chickens. Conditionally pathogenic bacteria, such as Proteus mirabilis, Klebsiella oxytoca, Klebsiella pneumoniae, Staphylococcus aureus were present. Bacillis spp. was present in all of the samples. After feeding the formulations, no pathogenic microflora was detected in the droppings of chickens in experiment groups. Conditionally pathogenic bacteria Proteus mirabilis were present in the droppings of control group chickens, the 1st experimental group, 2nd experimental group. Concentrations of Bifidobacteria and Lactobacteria in chickens’ droppings fed with “Lactobifadol” were significantly higher than in the rest of the groups. Bacillus spp. was present in all samples of the 1st experimental group fed with “Vetom 2”. It was also present in the droppings of chickens in other groups. Scientific novelty. Laboratory experiment involving broiler chickens aimed at researching intestinal microbiocenosis and its effect on digestibility of mixed fodder was conducted in Northern Trans-Urals conditions for the first time.
broiler chickens, Vetom, Lactobifadol, intestinal microbiocenosis, digestibility coefficients
1. Samigulina S. I. Farmakologicheskie aspekty primeneniya veterinarnykh immunostimuliruyushchikh preparatov [Pharmacological aspects of the use of veterinary immunostimulating formuations] // Youth and science. 2019. No. 2. Article number. 35. (In Russian.)
2. Glaskovich M. A., Marashchuk Yu. V. Ekologicheskaya bezopasnost’ proizvodstva produktsii sel’skogo khozyaystva pri vvedenii v ratsion immunostimuliruyushchego probiotikosoderzhashchego kompleksa biologicheski aktivnykh veshchestv [Environmental safety of producing agricultural food productsafter introduction an immunostimulating probiotic-containing complex of biologically active substances into the diet] // Materialy Nauchno-prakticheskoy konferentsii KF RGAU-MSKhA imeni K. A. Timiryazeva. Kaluga, 2018. Pp. 26-29. (In Russian.)
3. Koshchaev A. G., Shchukina I. V., Garkovenko A. V., Ilnitskaya E. V., Radchenko V. V., Bakharev A. A., Khrabrova L. A. Allelic variation of marker genes of hereditary diseases and economically important traits in dairy breeding cattle population // Journal of Pharmaceutical Sciences and Research. 2018. No. 10 (6). Pp. 1566-1572.
4. Antipov A. A., Fisinin V. I., Egorov I. A. Effektivnost’ primeneniya probiotika OLIN pri vyrashchivanii tsyplyat-broylerov Efficiency of using OLIN probiotic in rearing of chicken-broilers // Zootekhnia. 2011. No. 1. Pp. 18-20. (In Russian.)
5. Natsional’nyy reestr pravovykh aktov Respubliki Belarus’ [National Register of Legal Acts of the Republic of Belarus]. 2021. No. 29, 5/33370. (In Russian.)
6. Shchepetkina S. V., Rishko O. A. Tekhnologiya vvoda probiotikov v tekhnologicheskiy tsikl ptitsevodcheskikh predpriyatiy [Procedure for introducing probiotics into the technological cycle of poultry enterprises] // Aktualnye voprosy selskohozyaistvennoi biologii. 2017. No. 4 (6). Pp. 49-57. (In Russian.)
7. Duktov A. P., Krasko P. A., Sadomov N. A. Bakharev A. A. et al. Ispol'zovanie immunomodulyatorov v broylernom ptitsevodstve: monografiya [The use of immunomodulators in broiler poultry farming: monograph]. Tyumen: State Agrarian University of the Northern Trans-Urals, 2021. 354 p. (In Russian.)
8. Shchepetkina S. V., Rishko O. A. Tselesoobraznost’ primeneniya probiotikov u raznykh vidov zhivotnykh [Rationale for using probiotics in different species of animals] // Innovations in Agricultural Complex: problems and perspectives. 2017. No. 4 (16). Pp. 2003-2012. (In Russian.)
9. Zakharov K. V., Ufimtsev N. A., Koninskiy V. S. Vozmozhnost’ ispol’zovaniya bakteriy roda Bacillus dlya proizvodstva dezinfektantov [The possibility of using Bacillus type bacteria for production of disinfectants] // Izvestiya Rossiiskoi voenno-medicheskoy akademii. 2018. Vol. 37. No. 1. S1-1. Pp. 226-228. (In Russian.)
10. Rimareva L. V., Volkova G. S., Kuksova E. V., Krivova A. Yu. Aspekty sozdaniya konsortsiuma mikroorganizmov, obladayushchego osobennostyami probiotika, dlya korrektsii disbioticheskikh narusheniy [Aspects of creating a consortium of microorganisms with probiotic features, for the therapy of dysbiotic disorders] // Storage and processing of farm products. 2017. No. 1. Pp. 39-44. (In Russian.)
11. Ignachenko V. E., Gordeeva A. K. Vliyanie probiotika Laktobifadola na produktivnye pokazateli broylerov v OOO “Sayanskiy broiler” [The influence of Lactobifadol probiotic on productive indicators of broilers at Sayan Broiler LLC] // Nauchnye issledovaniya i razrabotki k vnedreniyu v APK: materialy mezhdunarodnoy nauchno-prakticheskoy konferentsii molodykh uchenykh. Molodezhnyy, 2020. Pp. 206-213. (In Russian.)
12. Glaskovich S. A. Krasochko P. P. Vlyanie probiotika “bifidoflorin zhidkiy” na kishechnyy biotsenoz i produktivnost’ tsyplyat-broylerov krossa SOVV-500 [Influencing of "liquid bifidofloran" probioticupon intestinal biocenosis and productivity of SOVV-500 breed broiler chickens] // Aktual’nye problemy intensivnogo razvitiya zhivotnovodstva, materialy KhVI Mezhdunarodnoy studencheskoy nauchnoy konferentsii, posvyashchennoy 80-letiyu kafedry razvedeniya i genetiki sel’skokhozyaystvennykh zhivotnykh UO “BGSKhA” Gorky, 2013. Pp. 37-40. (In Russian.)
13. Nikolaenko E. I., Lukina D. V., Glebova I. V. Primenenie probiotikov v kormlenii tsyplyat-broylerov [The use of probiotics in the feeding of broiler chickens] // Molodezhnaya nauka - garant innovatsionnogo razvitiya APK, materialy X Vserossiyskoy (natsional’noy) nauchno-prakticheskoy konferentsii studentov, aspirantov i molodykh uchenykh. Kursk, 2019. Pp. 123-127. (In Russian.)
14. Aleksandrova S., Simonov O. et al. Silver in the meat and organs of broiler chickens in case of using colloidal silver as an alternative to antibiotics // BioMetals. 2018. No. 31 (6). Pp. 975-980. DOI:https://doi.org/10.1007/s10534-018-0141-3.
15. Kannan G. Elevated plasma corticosterone concentrations influence the onset of rigor mortis and meat color in broilers // Poultry Science. 2018. Vol. 77. Pp. 322-326. DOI:https://doi.org/10.1093/ps/77.2.322.
16. Aleksandrova S. S., Bakharev A. A., Simonov O. A., Renev E. P. et al. Kharakteristika produktivnykh i gematologicheskikh pokazateley tsyplyat-broylerov pri ispol’zovanii v ikh vyrashchivanii raznykh antimikrobnykh veshchestv [Distinguishing characteristic features of productive and hematological indicators of broiler chickens when using various antimicrobial substances during their rearing] // Feeding of Agricultural Animals and Feed Production. 2020. No. 5. Pp. 25-44. DOI:https://doi.org/10.33920/sel-05-2005-04. (In Russian.)
17. Babin G. Yu., Polunochkina T. V., Dorofeeva S. G., Aleksandrova S. S., Miftakhutdinov A. V. Sokhranenie proizvodstvennykh pokazateley u tsyplyat-broylerov v usloviyakh teplovogo stressa [Preserving performance indicators in broiler chickens under conditions of thermal stress] // Agrarian science. 2022. No. 1. Pp. 19-23. DOI:https://doi.org/10.32634/0869-8155-2022-355-1-19-23. (In Russian.)
18. Rafikova E. R., Nozdrin G. A. Vliyanie preparata “Vetom” na osnove Duddingtonia flagrans na intensivnost’ rosta tsyplyat-broylerov [Influence of “Vetom” formulation based on Duddingtonia flagrans on growth intensity of broiler chickens] // Teoriya i praktika sovremennoi agrarnoi nauki: Sbornik IV natsional'noy (vserossiyskoy) nauchnoy konferentsii s mezhdunarodnym uchastiem Novosibirsk. 2018. Pp. 444-446. DOI:https://doi.org/10.31677/2072-6724-2018-48-3-92-102. (In Russian.)
19. Shul’ga N. N., Shul’ga I. S., Plavshak L. P., Dikunina S. S. Al’ternativnaya antibiotikam terapiya zabolevaniy zhivotnykh preparatom “Vetom 1.1” [Alternative antibiotic therapy of animal diseases with “Vetom 1.1” formulation] // Tendentsii razvitiya nauki i obrazovaniya. 2020. No. 58-3. Pp. 46-50. DOI:https://doi.org/10.18411/lj-02-2020-47. (In Russian.)
