Russian Federation
Yekaterinburg, Russian Federation
Yekaterinburg, Russian Federation
Yekaterinburg, Russian Federation
Abstract. The study of phytobiotics application is one of the topical directions in the strategy of antimicrobial resistance containment. The aim of this work was to study the effects of separate and combined use of enrofloxacin and phytobiotic drugs in 400-day-old laying hens. Methods. The studies were conducted on four groups of Loman-classic layers; in the course of the experiment, enrofloxacin and a phytobiotic based on sea buckthorn cake and mustard, cedar and sea buckthorn oils were introduced into the diet. Results. It was found that the use of drugs had no significant effect on the composition of microflora and the level of antibiotic resistance of gastrointestinal bacteria in chickens because by the age of 400 days the intestinal microbiomes of laying hens were already formed, stable and contaminated with AMR agents. Also when combining antibiotic and phytobiotic there was an increase in egg laying rate, average egg weight, accelerated excretion of enrofloxacin from the body of layers and reduction of its content in the egg to the detection limit. While in the group of laying hens receiving only enrofloxacin, its residual content in the egg was detected 2 weeks after cessation of its introduction. Thus, in the course of this research, the most optimal was the combined use of enrofloxacin with a phytobiotic drug, which had the most noticeable positive effect on metabolic parameters, productivity and egg quality. The novelty of the work is to obtain data on the effect of phytobiotic drug on a number of health indicators, productivity of laying hens and egg quality, and the selection of promising scheme of its use.
phytobiotic, productivity, laying hens, eggs, resistance genes, antibiotic susceptibility, enrofloxacin, E. coli, opportunistic pathogenic microflora
1. Vasil'eva O. A., Nufer A. I., Shackih E. V. Al'ternativnye puti zameny kormovyh antibiotikov // Effektivnoe zhivotnovodstvo. 2019. № 4 (152). S. 13-15.
2. Strel'nikova I. I., Kislicyna N. A. Effektivnost' primeneniya fitobiotikov v pticevodstve // Vestnik Mariyskogo gosudarstvennogo universiteta. Seriya «Sel'skohozyaystvennye nauki. Ekonomicheskie nauki». 2020. № 4 (24). C. 433-444. DOI:https://doi.org/10.30914/2411-9687-2020-6-4-433-444.
3. Sultanaeva L. Z., Baldzhi Yu. A. Effektivnost' ispol'zovaniya fitobioticheskih dobavok v racione krupnogo i melkogo rogatogo skota (obzor) // Zhivotnovodstvo i kormoproizvodstvo. 2021. № 2. S. 96-110. DOI:https://doi.org/10.33284/2658-3135-104-2-96.
4. Selivanova Yu. A. Shirokiy spektr fitoncidov - maksimal'naya funkcional'nost' fitobiotika // Pticevodstvo. 2018. № 1. S. 37-40.
5. Yamashiro Y. Gut Microbiota in Health and Disease // Annals of Nutrition and Metabolism. 2017. Vol. 71. No. 3-4. Pp. 242-246. DOI:https://doi.org/10.1159/000481627.
6. Kraimi N., Dawkins M., Gebhardt-Henrich S.G. Influence of the Microbiota-Gut-Brain Axis on Behavior and Welfare in Farm Animals: A Review // Physiology and Behavior. 2019. Vol. 210. Article number 112. DOI:https://doi.org/10.1016/j.physbeh.2019.112658.
7. Guitton E., Faurie A., Lavillatte S. Production of Germ-Free Fast-Growing Broilers from a Commercial Line for Microbiota Studies // Journal of Visualized Experiments. 2020. Vol. 18. Pp. 61-148. DOI:https://doi.org/10.3791/61148.
8. Bagno O. A., Prohorov O. N., Shevchenko S. A. [i dr.] Fitobiotiki v kormlenii sel'skohozyaystvennyh zhivotnyh // Sel'skohozyaystvennaya biologiya. 2018. T. 53. -№ 4. S. 687-697. DOI:https://doi.org/10.15389/agrobiology.2018.4.687rus.
9. Zinnatov F. F., Shamsova A. R., Zinnatova F. F., Ahmetov T. M., Safiullina A. R. Vzaimosvyaz' polimorfizma genov lipidnogo obmena (Lep, tg5) s molochnoy produktivnost'yu krupnogo rogatogo skota // Uchenye zapiski KGAVM im. N. E. Baumana. 2017. № 3. Pp. 72-75.
10. Vertiprahov V. G., Egorov I. A., Lenkova T. N. [i dr.] Ispol'zovanie fitobiotika i probiotika v kombikormah dlya myasnyh kur selekcii SGC «Smena» // Veterinariya i kormlenie. 2020. № 6. S. 7-12. DOI:https://doi.org/10.30917/ATT-VK-1814-9588-2020-6-2.
11. Nuraliev E. R., Kochish I. I. Primenenie fitobiotika «Provitol» dlya uluchsheniya konversii korma v promyshlennom pticevodstve // Vestnik AGAU. 2017. № 8 (154). S. 112-117.
12. Shackih E. V., Latypova E. N., Nesvat E. G., Polyakov P. S. Produktivnost' kur-nesushek pri vklyuchenii v racion fitobiotikov // Pticevodstvo. 2021. № 6. S. 35-39. DOI:https://doi.org/10.33845/0033-3239-2021-70-6-35-39.
13. Kothari D., Lee W.-D., Niu K.-M., Kim S.-K. The genus Allium as poultry feed additive: A review // Animals. 2019. Vol. 9. Article number 1032. DOI:https://doi.org/10.3390/ani9121032.
14. Abad P., Arroyo-Manzanares N., Ariza J. J., Baños A., García-Campaña A. M. Effect of Allium extract supplementation on egg quality, productivity, and intestinal microbiota of laying hens // Animals. 2020. Vol. 41. Pp. 48-54. DOI:https://doi.org/10.3390/ani11010041
15. Rabelo-Ruiz M., Ariza-Romero J. J., Zurita-González M. J., Martín-Platero A. M., Baños A., Maqueda M., Valdivia E., Martínez-Bueno M., Peralta-Sánchez J. M. Allium-Based Phytobiotic Enhances Egg Production in Laying Hens through Microbial Composition Changes in Ileum and Cecum // Animals (Basel). 2021. No. 11 (2). Article number 448. DOI:https://doi.org/10.3390/ani11020448.
16. Khan S., Moore R. J., Stanley D., Chousalkar K. K. The Gut Microbiota of Laying Hens and Its Manipulation with Prebiotics and Probiotics to Enhance Gut Health and Food Safety // Applied and Environmental Microbiology. 2020. Vol. 86 (13). Pp. 5-10. DOI:https://doi.org/10.1128/AEM.00600-20.
17. Tzeng T. J., Liu T. Y., Lin C. W. et al. Effects of Dietary Inclusion of Dry Hydrastis Canadensis on Laying Performance, Egg Quality, Serum Biochemical Parameters and Cecal Microbiota in Laying Hens // Animals (Basel). 2021. No. 11 (5). Article number 1381. DOI:https://doi.org/10.3390/ani11051381.
18. Abd El-Hack M. E., Alagawany M., Abdel-Moneim A. E. et al. Cinnamon (Cinnamomum zeylanicum) Oil as a Potential Alternative to Antibiotics in Poultry // Antibiotics (Basel). 2020. No. 9 (5). Article number 210. DOI:https://doi.org/10.3390/antibiotics9050210.
