IDENTIFICATION OF SNPS ASSOCIATED WITH THE REPRODUCTIVE ABILITY OF CATTLE
Rubrics: BIOLOGY
Abstract and keywords
Abstract (English):
The problem of reproduction of cattle in the Russian Federation remains relevant from year to year and is characterized by a rather low yield of offspring per 100 queens, which causes significant economic damage. The solution to this problem is associated with an increase in the efficiency of the implementation of the genetically determined reproductive capabilities of animals. Scientific novelty lies in the study of molecular genetic mechanisms of cattle fertility control. The aim of the work was to identify allelic variants of SNPs associated with the reproductive ability of cattle. Whole genome genotyping was performed. Research methods. The studies were carried out on cows of the Holsteinized Black-and-White breed of the Ural type. The genetic profile of Black-and-White animals has been studied, SNP significantly associated with the reproductive qualities of cows have been identified, genetic variants and combinations of DNA markers have been identified that improve the reproductive qualities of cows. Results. In terms of reproduction indicators (multiplicity of insemination, duration of the service period), it was found that the multiplicity of insemination for animals with the genotype ARS-BFGL-NGS-71623GG was 1.33, which is lower compared to the genotypes ARS-BFGL-NGS-71623AA and ARS-BFGL- NGS-71623AG by 0.27 and 0.5 units. Heifers with the ARS-BFGL-NGS-85003AC genotype are inseminated at an earlier age than other animals. A significant difference in live weight at the first insemination was noted only for the ARS-BFGL-NGS-71623 gene. It is recommended to select animals with homozygous genotype ARS-BFGL-NGS-71623GG to improve the reproductive ability of cows in the breeding nucleus.

Keywords:
cattle, whole genome genotyping, reproductive ability, DNA markers
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References

1. Getmanceva L. V., Shevcova V. S., Kolosova M. A., Romanenkova O. S., Kostyunina O. V. Issledovanie gaplotipov fertil'nosti u golshtinskih korov gollandskogo proishozhdeniya v usloviyah rostovskoy oblasti // Glavnyy zootehnik. 2020. № 4. S. 36-40.

2. Fornara M. S., Kostyunina O. V., Filipchenko A. A., Sermyagin A. A., Zinov'eva N. A. Sistema opredeleniya polimorfizma SUGT1, associirovannogo s gaplotipom fertil'nosti simmental'skogo skota FH4 // Veterinariya, zootehniya i biotehnologiya. 2019. № 3. S. 92-97. DOI:https://doi.org/10.26155/vet.zoo.bio.201903015.

3. Isupova Yu. V., Achkasova E V. Perspektivy ispol'zovaniya ocenki genomnoy plemennoy cennosti v selekcii molochnogo skota v usloviyah Udmurtskoy Respubliki // Izvestiya Orenburgskogo gosudarstvennogo agrarnogo universiteta. 2021. № 4 (90). S. 307-311.

4. Naryshkina E. N., Sermyagin A. A. Ocenka geneticheskoy i genomnoy variabel'nosti priznakov fertil'nosti bykov-proizvoditeley na osnove lokusov v genome, associirovannyh s davleniem otbora (obzor) // Dostizheniya nauki i tehniki APK. 2020. № 9. S. 64-72.

5. Romanenkova O. S., Volkova V. V., Kostyunina O. V., Zinov'eva N. A. Issledovanie Rossiyskoy populyacii golshtinskogo i golshtinizirovannogo cherno-pestrogo kupnogo rogatogo skota na nalichie mutacii v gene TFB1M, associirovannogo s gaplotipom HH5 // Biotehnologiya v rastenievodstve, zhivotnovodstve i sel'skohozyaystvennoy mikrobiologii: sbornik tezisov dokladov 19-y Vserossiyskoy konferencii molodyh uchenyh, posvyaschennoy pamyati akademika RASHN Georgiya Sergeevicha Muromceva. Moskva, 2019. S. 119-120.

6. Kuznecova M. K., Kislyakova E. M., Isupova Yu. V. Dostovernost' ucheta dannyh kak odin iz sposobov povysheniya tochnosti pri ocenke plemennoy cennosti // Agrarnaya Rossiya. 2022. № 1. S. 27-30.

7. Luk'yanov A. A., Tyulebaev S. D., Kosilov V. I. Ispol'zovanie vozmozhnostey genomnoy ocenki krupnogo rogatogo skota v RF // Aktual'nye problemy veterinarnoy mediciny i zootehnii: sbornik materialov Nacional'noy nauchno-prakticheskoy konferencii s mezhdunarodnym uchastiem, posvyaschennoy 80-letiyu doktora sel'skohozyaystvennyh nauk, professora kafedry veterinarno-sanitarnoy ekspertizy i farmakologii FGBOU VO Orenburgskiy GAU Lyapina Olega Abdulhakovicha. Orenburg, 2022. S. 132-137.

8. Abdel'manova A. S., Sermyagin A. A., Docev A. V., Rodionov A. N., Stolpovskiy Yu. A., Zinov'eva N. A. Polnogenomnye issledovaniya struktury populyaciy rossiyskih lokal'nyh porod cherno-pestrogo kornya // Genetika. 2022. T. 58. № 7. S. 786-797. DOI:https://doi.org/10.31857/S0016675822070025.

9. Ganiev A. S., Sibagatullin F. S., Shaydullin R. R., Faizov T. H. Servis-period i molochnaya produktivnost' korov s raznymi genotipami CSN3 i DGAT1 // Uchenye zapiski KGAVM im. N. E. Baumana. 2018. № 2. S. 67-72.

10. Ivanova I. P., Trocenko I. V. Primenenie selekcionno-geneticheskih parametrov v plemennoy rabote s molochnym skotom // Vestnik KrasGAU. 2019. № 3 (144). S. 65-70.

11. Yakovlev A. F. Vklad gaplotipov v formirovanie plemennyh i vosproizvoditel'nyh kachestv zhivotnyh (obzor) // Problemy biologii produktivnyh zhivotnyh. 2019. № 2. S. 5-18. DOI:https://doi.org/10.25687/1996-6733.prodanimbiol.2019.2.5-18.

12. Guarini A. R., Lourenco D. A. L., Brito L. F., Sargolzaei M., Baes C. F., Miglior F., Misztal I., Schenkel F. S. Genetics and genomics of reproductive disorders in Canadian Holstein cattle // Journal of Dairy Science. 2019. No. 102. Iss. 2. Rp. 1341-1353. DOI:https://doi.org/10.3168/jds.2018-15038.

13. Garcia A. O., Otto P. I., Glatzl Junior L. A., Rocha R. F. B., Dos Santos M. G., de Oliveira D. A., da Silva M. V. G. B., Panetto J. C. D. C., Machado M. A., Verneque R. D. S., Guimarães S. E. F. Pedigree reconstruction and population structure using SNP markers in Gir cattle // Journal of Applied Genetics. 2023. No. 64 (2). Pp. 329-340. DOI:https://doi.org/10.1007/s13353-023-00747-x.

14. Ma L., Cole J. B., Da Y., VanRaden P. M. Genetics, genome-wide association study, and genetic improvement of dairy fertility traits // Journal of Dairy Science. 2019. Vol. 102. Iss. 4. Rp. 3735-3743. DOIhttps://doi.org/10.3168/jds.2018-15269.

15. Huang M., Liu X., Zhou Y., Summers R. M., Zhang Z. BLINK: a package for the next level of genome-wide association studies with both individuals and markers in the millions // Gigascience. 2019. No. 8 (2). Article number giy154. DOI:https://doi.org/10.1093/gigascience/giy154.

16. Pausch H., Schwarzenbacher H., Burgstaller J., Flisikowski K., Wurmser K., Jansen S., Jung S., Schnicke A., Wittek T., Rudy K. Homozygous haplotype deficiency reveals deleterious mutations compromising reproductive and rearing success in cattle // BMC Genomics. 2015. No. 16 (1). Article number 312. DOI:https://doi.org/10.1186/S12864-015-1483-7.

17. Wang J., Zhou Z., Zhang Z., Li H., Liu D., Zhang Q., Bradbury P. J., Buckler E. S., Zhang Z. Expanding the BLUP alphabet for genomic prediction adaptable to the genetic architectures of complex traits // Heredity. 2018. Vol. 121 (6). Pp. 648-662. DOI:https://doi.org/10.1038/s41437-018-0075-0.

18. Sahana G., Nielsen U. S., Aamand G. P., Lund M. S., Guldbrandtsen B. Novel Harmful Recessive Haplotypes Identified for Fertility Traits in Nordic Holstein Cattle // PLoS ONE. 2013. Vol. 8. Iss. 12. Article number 82909. DOI:https://doi.org/10.1371/journal.pone.0082909.

19. Sugimoto M., Gotoh Y., Kawahara T., Sugimoto Y. Molecular Effects of Polymorphism in the 3’UTR of Unc-5 homolog C Associated with Conception Rate in Holsteins // PLoS ONE. 2015. Vol. 10. Iss. 7. Article number 0131283. DOI:https://doi.org/10.1371/journal.pone.0131283.

20. VanRaden M., Olson K. M., Null D. J., Hutchison J. L. Harmful recessive effects on fertility detected by absence of homozygous haplotypes // Journal of Dairy Science. 2011. Vol. 94. Iss. 12. Rp. 6153-6161. DOI:https://doi.org/10.3168/JDS.2011-4624.

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