Abstract. Potatoes with pigmented tubers contain anthocyanins and other phenolic compounds that demonstrate antioxidant activity and have a beneficial effect on human health. The nutritional value and other consumer qualities of available potato varieties must be combined with resistance to various infections, primarily late blight, which leads to significant yield losses and considerable costs for chemical protection. The purpose of this work was to assess late blight resistance of 46 promising potato hybrids with different pigmentation patterns, both in the field and by laboratory methods, including molecular screening. The objectives of this study included obtaining promising hybrids of colored potatoes characterized by a set of economically importatnt traits, laboratory and field assessment of the resistance of leaves and tubers to the late blight pathogen Phytophthora infestans, as well as molecular screening of late blight resistance gene markers (Rpi genes). The production, cultivation and evaluation of the hybrid material was carried out using standard methods. Field and laboratory resistance of leaves and tubers was assessed on a 9-point scale. Molecular screening was carried out using PCR analysis. The scientific novelty of the work lies in the fact that for the first time a comprehensive assessment of the resistance to late blight of hybrid material of colored potatoes obtained on the territory of Russian Federation was carried out. According to the results obtained, the studied samples demonstrated a high level of resistance of tubers and leaves in field conditions and laboratory tests. However, the results of the study did not reveal a relationship between laboratory or field resistance and the presence of Rpi gene markers, which may be due to the influence of many independent factors that determine horizontal resistance.
potato, late blight, horizontal resistance, Rpi genes, molecular markers, anthocyanins
1. Eichhorn C., Winterhalter P, Anthocyanins from pigmented potato (Solanum tuberosum L.) varieties // Food Research International. 2005. No. 38. Pp. 943–948. DOI:https://doi.org/10.1016/j.foodres.2005.03.011.
2. Polivanova O. B., Gins E. M. Antioksidantnaya aktivnost' pigmentirovannogo kartofelya (Solanum tuberosum L.), soderzhanie antocianov, ih biosintez i fiziologicheskaya rol' // Ovoschi Rossii. 2019. № 6 (50). S. 84–90. DOI:https://doi.org/10.18619/2072-9146-2019-6-84-90.
3. Jo K. R., Kim C. J, Kim S. J., Kim T. Y., Bergervoet M., Jongsma M. A., Visser R. G., Jacobsen E., Vossen J. H. Development of late blight resistant potatoes by cisgene stacking // BMC Biotechnology. 2014. No. 14. Article number 50. DOI:https://doi.org/10.1186/1472-6750-14-50.
4. Rogozina E. V., Gurina A. A., Chalaya N. A., Zoteyeva N. M., Kuznetsova M. A., Beketova M. P., Muratova O. A., Sokolova E. A., Drobyazina P. E., Khavkin E. E. Diversity of Late Blight Resistance Genes in the VIR Potato Collection // Plants. 2023. No. 12 (2). Article number 273. DOI:https://doi.org/10.3390/plants12020273.
5. Haverkort A. J., Boonekamp P. M., Hutten R., Jacobsen E., Lotz L. A. P., Kessel G. J. T., Vossen J. H., Visser R. G. F. Durable late blight resistance in potato through dynamic varieties obtained by cisgenesis: scientific and societal advances in the DuRPh project // Potato Research. 2016. No. 59. Pp. 35–66. DOI:https://doi.org/10.1007/s11540-015-9312-6.
6. Paluchowska P., Śliwka J., Yin Z. Late blight resistance genes in potato breeding // Planta. 2022. No. 255. Article number 127. DOI:https://doi.org/10.1007/s00425-022-03910-6.
7. Meade F., Hutten R., Wagener S., Prigge V., Dalton E., Kirk H. G., Griffin D., Milbourne D. Detection of novel QTLs for late blight resistance derived from the wild potato species Solanum microdontum and Solanum pampasense // Genes. 2020. No. 11 (7). Article number 732. DOI:https://doi.org/10.3390/genes11070732.
8. Tiwari J. K., Rawat S., Luthra S. K., Zinta R., Sahu S., Varshney S., Kumar V., Dalamu D., Mandadi N., Kumar M., Chakrabarti S. K., Rao A. R., Rai A. Genome sequence analysis provides insights on genomic variation and late blight resistance genes in potato somatic hybrid (parents and progeny) // Molecular Biology Reports. 2021. No. 48 (1). Pp. 623–635. DOI:https://doi.org/10.1007/s11033-020-06106-x.
9. Kieu N. P., Lenman M., Wang E. S., Petersen B. L., Andreasson E. Mutations introduced in susceptibility genes through CRISPR/Cas9 genome editing confer increased late blight resistance in potatoes // Scientific Reports. 2021. No. 11. Article number 4487. DOI:https://doi.org/10.1038/s41598-021-83972-w.
10. Hegde N., Joshi S., Soni N., Kushalappa A. C. The caffeoyl-CoA O-methyltransferase gene SNP replacement in Russet Burbank potato variety enhances late blight resistance through cell wall reinforcement // Plant Cell Reports. 2021. No. 40 (1). Pp. 237–254. DOI:https://doi.org/10.1007/s00299-020-02629-6.
11. Angmo D., Sharma S. P., Kalia A. Breeding strategies for late blight resistance in potato crop: recent developments // Molecular Biology Reports. 2023. No. 50 (9). Pp. 7879–7891. DOI:https://doi.org/10.1007/s11033-023-08577-0.
12. Duan Y., Duan S., Xu J., Zheng J., Hu J., Li X., Li B., Li G., Jin L. Late blight resistance evaluation and genome-wide assessment of genetic diversity in wild and cultivated potato species // Frontiers in Plant Science. 2021. No. 12. Article number 710468. DOI:https://doi.org/10.3389/fpls.2021.710468.
13. Cucak M., de Andrade Moral R., Fealy R., Lambkin K., Kildea S. Opportunities for improved potato late blight management in the republic of ireland: field evaluation of the modified irish rules crop disease risk prediction model // Phytopathology. 2021. No. 111 (8). Pp. 1349–1360. DOI:https://doi.org/10.1094/PHYTO-01-20-0011-R.
14. Dalamu, Tiwari J. K., Bairwa A., Bhatia N., Zinta R., Kaushal N., Kumar V., Sharma A. K., Sharma S., Choudhary B., Luthra S. K., Buckseth T., Singh R. K., Thakur A. K., Kumar M., Kumar D. Resistance evaluation for native potato accessions against late blight disease and potato cyst nematodes by molecular markers and phenotypic screening in India // Life. 2022. No. 13 (1). Article number 33. DOI:https://doi.org/10.3390/life13010033.
15. Perez W., Alarcon L., Rojas T., Correa Y., Juarez H., Andrade-Piedra J. L., Anglin N. L., Ellis D. Screening south american potato landraces and potato wild relatives for novel sources of late blight resistance // Plant Disease. 2022. No. 106 (7). Pp. 1845–1856. DOI:https://doi.org/10.1094/PDIS-07-21-1582-RE.
16. Dzedaev H. T., Gazdanova I. O., Bekmurzov B. V. Biologicheskaya bor'ba s fitoftorozom kartofelya, vyzyvaemym Phytophthora infestans // Agrarnyĭ vestnik Urala. 2023. T. 23, № 09. S. 2‒10.
17. Simakov E. A. Sklyarova N. P., Yashina I. M. Metodicheskie ukazaniya po tehnologii selekcionnogo processa kartofelya. Moskva: Dostizheniya nauki i tehniki APK, 2006. 70 s.
18. Barkov V. A. Metodika issledovaniy po zaschite kartofelya ot bolezney, vrediteley, sornyakov i immunitetu. Moskva: VNIIKH, 1995. 108 s.
19. Zoteeva N., Zimnoh-Guzovska E. Novyy metod ocenki ustoychivosti klubney kartofelya k fitoftorozu // Mikologiya i fitopatologiya. 2004. T. 38, № 1. S. 89–93.
20. Blatnik E., Horvat M., Berne S., Humar M., Dolničar P., Meglič V. Late blight resistance conferred by Rpi-smira2/r8 in potato genotypes in vitro depends on the genetic background // Plants. 2022. No. 11 (10). Article number 1319. DOI:https://doi.org/10.3390/plants11101319.
21. Sokolova E., Pankin A., Beketova M., Kuznetsova M., Spiglazova S., Rogozina E., Yashina I., Khavkin E. SCAR markers of the R-genes and germplasm of wild Solanum species for breeding late blight-resistant potato cultivars // Plant Genetic Resources. 2011. No. 9 (2). Pp. 309–312. DOI:https://doi.org/10.1017/S1479262111000347.
22. Kim H. J., Lee H. R., Jo K. R. et al. Broad spectrum late blight resistance in potato differential set plants MaR8 and MaR9 is conferred by multiple stacked R genes // Theoretical and Applied Genetics. 2012. No. 124. Pp. 923–935. DOI:https://doi.org/10.1007/s00122-011-1757-7.
23. Wang M., Allefs S., van den Berg R. G., Vleeshouwers V. G., van der Vossen E. A., Vosman B. Allele mining in Solanum: conserved homologues of Rpi-blb1 are identified in Solanum stoloniferum // Theoretical and Applied Genetics. 2008. Vol. 116. Pp. 933–943. DOI:https://doi.org/10.1007/s00122-008-0725-3.
24. Rietman H., Bijsterbosch G., Cano L. M., Lee H. R., Vossen J. H., Jacobsen E., Visser R. G., Kamoun S., Vleeshouwers V. G. Qualitative and quantitative late blight resistance in the potato cultivar Sarpo Mira is determined by the perception of five distinct RXLR effectors // Molecular Plant-Microbe Interactions. 2012. No. 25 (7). Pp. 910–919. DOI:https://doi.org/10.1094/MPMI-01-12-0010-R.
25. Zhu S., Li Y., Vossen J. H., Visser R. F., Jacobsen E. Functional stacking of three resistance genes against Phytophthora infestans in potato // Transgenic Research. 2012. No. 21. Pp. 89–99. DOI:https://doi.org/10.1007/s11248-011-9510-1.
26. Sagai-Maroof M. A., Soliman K. M., Jorgensen R. A., Allard R. W. Ribosomal DNA spacer-length polymorphism in barley: mendelian inheritance, chromosomal location, and population dynamics // Proceedings of the National Academy of Sciences. 1984. No. 81. Pr. 8014–8018. DOI:https://doi.org/10.1073/pnas.81.24.8014.
27. Sharma B. P., Gregory A. F., Hira K. M., Sundar M. S., Resham B. T. Determination of Resistance to Phytophthora Infestans on Potato Plants in Field, Laboratory and Greenhouse Conditions // Journal of Agricultural Science. 2013. No. 5 (5). Pp. 148–157. DOI:https://doi.org/10.5539/jas.v5n5p148.
28. Budin K. Z. Geneticheskie osnovy selekcii kartofelya. Leningrad: Agropromizdat, 1986. S. 61–66.
29. Fadina O. A., Beketova M. P., Sokolova E. A., Kuznecova M. A., Smetanina T. I., Rogozina E. V., Havkin E. E. Uprezhdayuschaya selekciya: ispol'zovanie molekulyarnyh markerov pri sozdanii donorov ustoychivosti kartofelya (Solanum tuberosum L.) k fitoftorozu na osnove slozhnyh mezhvidovyh gibridov // Sel'skohozyaystvennaya biologiya. 2017. T. 52, № 1. S. 84–94. DOI:https://doi.org/10.15389/agrobiology.2017.1.84rus.
30. Fry W. E. Phytophthora infestans: New tools (and old ones) lead to new understanding and precision management // Annual Review of Phytopathology. 2016. No. 54. Pp. 529–547. DOI:https://doi.org/10.1146/annurev-phyto-080615-095951.
31. Gastelo M., Perez W., Quispe K. et al. Phenotypic stability and correlation for late blight resistance in advanced potato clones under field and controlled conditions // American Journal of Potato Research. 2022. No. 99. Pp. 150–159. DOI:https://doi.org/10.1007/s12230-022-09861-8.
32. Mahfouze H. A., El-Sayed O. E., Mahfouze S. A. Characterization of resistance genes to late blight (Phytophthora infestants) in potato by marker-assisted selection // Journal of Applied Biology & Biotechnology. 2023. No. 11 (2). Pp. 178–186. DOI:https://doi.org/10.7324/JABB.2023.110218.
