Abstract. The purpose of this study is to increase the efficiency of microbial composting of organic poultry waste and to ensure the preservation of nutrients in compost by using a functional complex of microbial destructor cultures integrated with a mineral carrier. Methods. Biomass of functional microorganisms was obtained by submerged cultivation and inoculated into a compostable substrate in a given chronological sequence. Three complexes of microorganisms were used (nitrifiers; thermophilic decomposers of cellulose and lignin of litter material; mesophilic microorganisms - soil formers). The functional group of nitrifiers was inoculated on a mineral carrier, glauconite, for the purpose of chemisorption of ammonia and its conversion into nitrates. Scientific novelty. Environmentally sound, similar to natural processes of destruction of organic matter and humification, methods of cascade destruction of cellulose- and lignin-containing waste by specially designed complexes of microorganisms, as well as the integration of microorganisms with a mineral carrier – glauconite – are fundamentally new approaches in the practice of bioconversion and the production of organomineral fertilizers. Practical significance. Using the described techniques in practice will reduce the emission of unwanted gases into the atmosphere, reduce nitrogen losses and obtain environmentally friendly compost, which can be used as an organomineral fertilizer. Results. In the dung and litter materials, the predominance of bacteria of the genera Bacillus and Enterobacter among other native microorganisms are initiators of the composting process was established. An increase in composting efficiency due to inoculation of complexes of functional microorganisms by 21.5 % was found. Nitrogen losses in the experiment were reduced by 30–33 %. Special techniques made it possible to bind residual amounts of ammonia and create favorable conditions for the activity of soil formers – actinomycetes at the final stages of destruction. The results obtained were achieved due to the optimal correspondence of the ecological and functional role of groups of microorganisms to each of the stages of substrate decomposition.
waste bioconversion, environmental safety, microbial fermentation, agricultural biotechnology
1. Yamaliev T. Sh., Bocharova A. A. Ekologicheskie problemy pticevodstva // Mir Innovaciy. 2021. № 4. S. 40–43.
2. Uktam T., Shafoat N., Akhmed R. Animal waste processing technology and poultry farming in organomineral fertilizers // International Journal of Psychosocial Rehabilitation. 2020. Vol. 24, No. 6. P. 7263-7274. DOI:https://doi.org/10.37200/IJPR/V24I6/PR260731.
3. Iljina G. V., Iljin D. Yu., Zimnyakov V. M., Sashenkova S. A. Influence of organomineral fertilizer based on fermented poultry waste on the physico-chemical parameters of agricultural soils // Scientific Papers. Series A. Agronomy. 2022. Vol. 65, No. 2. Pp. 91–96.
4. Harindintwali J. D., Zhou J., Muhoza B., Wang F., Herzberger A., Yu X. Integrated eco-strategies towards sustainable carbon and nitrogen cycling in agriculture // Journal of Environmental Management. 2021. Vol. 293. Article number 112856. DOI:https://doi.org/10.1016/j.jenvman.2021.112856.
5. Crippen T. L., Sheffield C. L, Singh B., Byrd J. A., Beier R. C., Anderson R. C. Poultry litter and the environment: Microbial profile of litter during successive flock rotations and after spreading on pastureland // Science of The Total Environment. 2021. Vol. 780. Article number 146413. DOI:https://doi.org/10.1016/j.scitotenv.2021.146413.
6. Li F., Ghanizadeh H., Cui G., Liu J., Miao S., Liu Ch., Song W., Chen X., Cheng M., Wang P., Zhang Y., Wang A. Microbiome – based agents can optimize composting of agricultural wastes by modifying microbial communities // Bioresource Technology. 2023. Vol. 374. Article number 128765. DOI:https://doi.org/10.1016/j.biortech.2023.128765.
7. Vorob'eva A. A. Il'ina G. V., Il'in D. Yu. Razrabotka tehnologii kaskadnoy mikrobnoy konversii othodov pticevodstva // Vklad molodyh uchenyh v innovacionnoe razvitie APK Rossii: sbornik materialov Vserossiyskoy (nacional'noy) nauchno-prakticheskoy konferencii molodyh uchenyh. Penza: Penzenskiy gosudarstvennyy agrarnyy universitet, 2021. T. I. S. 192–194.
8. Vorob'eva A. A. Proizvodstvo organomineral'nogo udobreniya putem biokonversii othodov pticevodstva // Innovacionnaya tehnika i tehnologiya. 2021. T. 8, № 2. S. 39–42.
9. Smirnov R. V., Bezdudnaya A. G, Treyman M. G. Innovacionnaya deyatel'nost' po pererabotke proizvodstvennyh othodov na primere pticefabriki // Tehniko-tehnologicheskie problemy servisa. 2020. № 1 (51). S. 54–60.
10. Fahreev N. N. Razrabotka i obosnovanie parametrov gazifikacionnoy ustanovki dlya utilizacii othodov pticevodstva: dis. … kand. tehn. nauk: 4.3.1. Kazan', 2023. 179 s.
11. Hoang H. G, Thuy B. T. P., Lin C., Vo D. N., Tran H. T., Bahari M. B., Le V. G., Vu C. T. The nitrogen cycle and mitigation strategies for nitrogen loss during organic waste composting: a review // Chemosphere. 2022. Vol. 300. Article number 134514. DOI:https://doi.org/10.1016/j.chemosphere.2022.134514.
12. Guo H., Zhao S., Xia D., Zhao W., Li Q., Liu X., Lv J. The biochemical mechanism of enhancing the conversion of chicken manure to biogenic methane using coal slime as additive // Bioresource Technology. 2022. Vol. 344 (Part B). Article number 126226. DOI:https://doi.org/10.1016/j.chemosphere.2022.134514.
13. Ravindran B., Karmegam N., Awasthi M. K., Chang S. W., Selvi P. K., Balachandar R., Chinnappan S., Azelee N. I. W., Munuswamy-Ramanujam G. Valorization of food waste and poultry manure through co-composting amending saw dust, biochar and mineral salts for value-added compost production // Bioresource Technology. 2022. Vol. 346. Article number 126442. DOI:https://doi.org/10.1016/j.biortech.2021.126442.
14. Sigurnjak I., Brienza C., Snauwaert E., De Dobbelaere A., De Mey J., Vaneeckhaute C., Michels E., Schoumans O., Adani F., Meers E. Production and performance of bio-based mineral fertilizers from agricultural waste using ammonia (stripping-)scrubbing technology // Waste Management. 2019. Vol. 89. Pp. 265–274. DOI:https://doi.org/10.1016/j.wasman.2019.03.043.
15. Esmaeilian Y., Amiri M. B., Tavassoli A., Caballero-Calvo A., Rodrigo-Comino J. Replacing chemical fertilizers with organic and biological ones in transition to organic farming systems in saffron (Crocus sativus) cultivation // Chemosphere. 2022. Vol. 307 (Part 1). Article number 135537. DOI:https://doi.org/10.1016/j.chemosphere.2022.135537.
16. Srivastava R. K., Shetti N. P., Reddy K. R., Aminabhavi T. M. Sustainable energy from waste organic matters via efficient microbial processes // Science of the Total Environment. 2020. Vol. 722. Article number 137927. DOI:https://doi.org/10.1016/j.scitotenv.2020.137927.
17. Pereira A. S. A. de P., Castro J. de S., Ribeiro V. J., Calijuri M. L. Organomineral fertilizers pastilles from microalgae grown in wastewater: Ammonia volatilization and plant growth // Science of The Total Environment. 2021. Vol. 779. Article number 146205. DOI:https://doi.org/10.1016/j.scitotenv.2021.146205.
18. Bibi F., Ilyas N., Arshad M., Khalid A., Saeed M., Ansar S., Batley J. Formulation and efficacy testing of bio-organic fertilizer produced through solid-state fermentation of agro-waste by Burkholderia cenocepacia // Chemosphere. 2022. Vol. 291 (Part 3). Article number 132762. DOI:https://doi.org/10.1016/j.chemosphere.2021.132762.
19. He Y., Zhang Y., Huang X., Xu J., Zhang H., Dai X., Li X. Deciphering the internal driving mechanism of microbial community for carbon conversion and nitrogen fixation during food waste composting with multifunctional microbial inoculation // Bioresource Technology. 2022. Vol. 360. Article number 127623. DOI:https://doi.org/10.1016/j.biortech.2022.127623.
20. Belov A. A., Chepcov V. S., Lysak L. V. Metody identifikacii pochvennyh mikroorganizmov. Moskva : OOO «MAKS Press», 2020. 196 s.
21. Mannapova R. T. Mikrobiologiya. Moskva: OOO «Prospekt», 2019. 440 s. DOI:https://doi.org/10.31085/9785392287734-2019-440.
