Abstract. Wild species of the genus Hemerocallis L. – family Hemerocallidaceae Br. – are of value as ornamental, food and medicinal plants. Biologically active substances of the species composition of lilies growing in natural habitats have not been studied enough. There is no information on the quantitative content of the main groups of biologically active substances in the five types of lilac cultivated in the forest-steppe zone of the Novosibirsk region over the two growing seasons, which determines the originality and significance of this work. The purpose of the study is a comparative study of the content of the main groups of biologically active substances (BAS) in plants of five species of lilac cultivated in the forest-steppe zone of Western Siberia. Methods. A spectrophotometric method was used to determine flavonols, catechins, carotenoids; carbazole-free – for the determination of pectins and protopectins; titrimetric – for the determination of tannins. Results. Comparative analysis of BAV in flowers and leaves of five species of lilac allowed to establish that in leaves the content of catechins (1.1–1.3 times), flavonols (2.0 times), tannins (1.6–2.3 times) is higher than in flowers. The content of pectins is higher in flowers (1.7–3.3 times), protopectins (1.4 times), carotenoids (3.7–34.4 times). The concentration of protopectins in leaves and flowers of all species is 1,4–5.1 times higher compared to pectins. H. minor (flavonols, pectins), H. middendorffii (tannins, catechins, carotenoids), H. citrina (catechins), H. fulva (tannins), H. lilio-asphodelus (pectins) were distinguished by high indications for individual components in various organs. Species specificity of accumulation of these components in flowers and leaves during the period of mass flowering and specificity of their content over two growing periods has been established. Scientific novelty. For the first time in the conditions of the forest-steppe zone of the Novosibirsk region, a comparative quantitative analysis of the content of phenolic compounds, pectin substances, carotenoids in the aboveground organs of H. lilio-asphodelus, H. minor, H. middendorffii, H. citrina, H. fulva during the growing season of 2019–2020 was carried out.
daylily, views, flower, leaf, tannins, flavonols, pectins, protopectins, catechins, carotenoids, forest-steppe zone, Novosibirsk region
1. Sedel'nikova L. L. Lileyniki v kollekcii Central'nogo sibirskogo botanicheskogo sada: biologiya i perspektivy // Subtropicheskoe i dekorativnoe sadovodstvo 2019. № 71. S. 109–116. DOI:https://doi.org/10.31360/2225-3068-2019-71-109-116.
2. Sedel'nikova L. L. Razvitie adaptirovannyh sortov Hemerocallis × hybrida v lesostepi Novosibirskoy oblasti // Vestnik Krasnoyarskogo gosudarstvennogo agrarnogo universiteta. 2021. № 8. S. 11—18. DOI: 10.367118/1819-4036-2021-8-11-18.
3. Li X., Jang S., Cui J., Qin X. Progress genus Hemerocallis in traditional uses, phytochemistry and pharmacology // The Journal of Horticultural Science and Biotechnology. 2021. Vol. 97, No. 3. DOI:https://doi.org/10.1080/14620316.2021.1988728.
4. Bzhickih N. V. Fertil'nost' sortov introducentov lileynika (Hemerocallidaceae) v usloviyah lesostepi Altayskogo kraya // Subtropicheskoe i dekorativnoe sadovodstvo. 2019. № 69. S. 31–39. DOIhttps://doi.org/10.31360/2225-3068-2019-69-31-39.
5. Bondorina I. A., Kabanov A. V., Mamaeva N. A., Hohlacheva Yu. A., Bumbeeva L. I. Sovremennoe sostoyanie kollekcionnogo fonda laboratorii dekorativnyh rasteniy GBS // Izvestiya Saratovskogo universiteta. Novaya seriya. Himiya. Biologiya. Ekologiya. 2019. T. 19, vyp. 1. S. 79–86.
6. Plugatar' Yu. V., Klimenko Z. K. Osnovnye principy kul'tivirovaniya cvetochno-dekorativnyh rasteniy v usloviyah Yuzhnogo berega Kryma // Byulleten' Gosudarstvennogo Nikitskogo botanicheskogo sada. 2019. Vyp. 133. S. 9–16. DOI:https://doi.org/10.36305/0513-1634-2019-133-9-16.
7. Seliverstova E. N., Schegrinec N. V. Bolezni i vrediteli lileynika gibridnogo (Hemerocallis) v Stavropol'skom botanicheskom sadu // Vestnik APK Stavropol'ya. 2020. T. 1, № 37. S. 71–74. DOI:https://doi.org/10.31279/2222-9345-2020-9-37-71-74
8. Seliverstova E. N. Introdukciya i akklimatizaciya sortov lileynika gibridnogo v Stavropol'skom botanicheskom sadu // Novosti nauki APK. 2019. № 1-1 (12). S. 113–115. DOI:https://doi.org/10.25930/fpac-wg17.
9. Okach M. A., Muhamedova S. V. Lileyniki kollekcii botanicheskogo sada-instituta PGTU // Vestnik PGTU. 2020. № 4 (48). S. 50–60. DOI:https://doi.org/10.25686/2306-2827.2020.4.50
10. Prihod'ko S. A., Makogon I. V. Kollekcii i ekspozicii cvetochno-dekorativnyh rasteniy Doneckogo botanicheskogo sada // Biologiya rasteniy i sadovodstvo: teoriya, innovacii. 2020. № 156. S. 28–36. DOI:https://doi.org/10.36305/2712-7788-2020-3-156-28-36
11. Antropova N. V. Prodolzhitel'nost' i produktivnost' elitnyh gibridov lileynika Hemerocallis L. v usloviyah lesostepi Altayskogo kraya // Vestnik Altayskogo gosudarstvennogo agrarnogo universiteta. 2021. № 9 (203). S. 25–29. DOI:https://doi.org/10.53083/1996-4277-2021-203-09-25-29.
12. Sedel'nikova L. L., Cheltygmasheva L. R. Anatomo-morfologicheskie osobennosti Hemerocallis minor (Hemerocallidaceae) v usloviyah lesostepi Novosibirskoy oblasti // Rastitel'nyy mir Aziatskoy Rossii. 2021. № 1. S. 54–65. DOI:https://doi.org/10.15372/RMAR20210105.
13. Sedelnikova L. Ontomorphogenesis of some species of Hemerocallis genus in the conditions of the forest-steppe zone of the Novosibirsk region // BIO Web of Conferences. 2021. Vol. 31. VI International Scientific Conference “Problems of Industrial Botany of Industrially Developed Regions” 2021. Article number 00025. DOI:https://doi.org/10.1051/bioconf/20213100025.
14. Pyatina I. S., Reut A. A., Zhigunova O. Yu., Kryukova A. V., Anischenko I. E., Shigalov Z. H. Kompleksnaya ocenka sortov lileynika gibridnogo v usloviyah lesostepnoy zony Bashkirskogo Predural'ya // Izvestiya TSHA. 2022. № 4. S. 33–47. DOI:https://doi.org/10.26897/0021-342H-2022-4-33-47.
15. Sedel'nikova L. L. Ranzhirovanie sortov lileynika gibridnogo kollekcii CSBS SO RAN i perspektivy racional'nogo ispol'zovaniya // Agrarnyy Vestnik Urala. 2022. № 9 (224). S. 47–58. DOI:https://doi.org/10.32417/1997-4868-2022-224-09-47-58.
16. Sedel'nikova L. L. Ocenka elementnogo sostava krasodneva kak bioindikatora ekologicheskogo sostoyaniya urbanizirovannoy sredy // Ekologiya i promyshlennost' Rossii. 2019. T. 23. № 5. S. 62–66. DOI:https://doi.org/10.18412/1816-0395-2019-05-62-66.
17. Sedel’nikova L. L., Chankina O. V. Elemental composition of the leaves and rhizomes Hemerocallis hybrida hort. // Chemistry for sustainable development. 2019. No. 27. Pp. 530–535. DOI:https://doi.org/10.15372/CSD2019170.
18. Sedel'nikova L. L., Candekova O. L. K specifike soderzhaniya himicheskih elementov i zol'nosti v list'yah travyanistyh rasteniy v usloviyah goroda Iskitim Novosibirskoy oblasti // Himiya rastitel'nogo syr'ya. 2021. № 1. S. 213–218. DOI:https://doi.org/10.14258/jcprm.2021018413.
19. Sedel'nikova L. L., Candekova O. L. Issledovanie biohimicheskogo sostava v list'yah predstaviteley roda Hemerocallis L. v usloviyah gorodov Novosibirskoy oblasti // Himiya rastitel'nogo syr'ya. 2022. № 3. S. 151–158. DOI:https://doi.org/10.14258/jcprm.20220311110.
20. Candekova O. L., Sedel'nikova L. L. Vliyanie urbanizirovannoy sredy na soderzhanie himicheskih elementov v list'yah dekorativnyh rasteniy // Ekologiya urbanizirovannyh territoriy. 2021. № 8. S. 6–10. DOI:https://doi.org/10.24412/1816-1863-2021-2-6-10.
21. Reut A. A. Soderzhanie biologicheski aktivnyh veschestv v introducirovannyh predstavitelyah roda Hemerocallis L. // Izvestiya Federal'nogo nauchnogo centra ovoschevodstva. 2019. № 1. S. 93–96.
22. Sedel'nikova L. L., Chankina O. V. Elementnyy sostav vegetativnyh organov sortov Regal Air i Speak to Me Hemerocallis hybrida // Himiya rastitel'nogo syr'ya. 2020. № 1. S. 245–250. DOI:https://doi.org/10.14258/jcprm.2020014612.
23. Pyatina I. S., Bastamova R. I., Reut A. A., Safiullina L. M., Shakurova E. R. Issledovanie elementnogo sostava rasteniy roda Hemerocallis L., proizrastayuschih na territorii respubliki Bashkortostan // Vestnik Bashkirskogo universiteta. 2021. T. 26, № 4. S. 944–949. DOI:https://doi.org/10.33184/bulletin-bsu-2021.4.14.
24. Sedel'nikova L. L., Kukushkina T. A. Fitohimicheskiy sostav v vegetativnyh organah Hemerocallis hybrida hort. ex Bergmans (Hemerocallidaceae) // Izvestiya Saratovskogo universiteta. Seriya: Himiya, biologiya, ekologiya. 2021. T. 21, vyp. 2. S. 203–210. DOI:https://doi.org/10.18500/1816-9775-2021-21-2-203-210.
25. Sedel'nikova L. L., Kukushkina T. A. Biologicheski aktivnye i zapasnye veschestva v vegetativnyh organah Hemerocallis hybrida sorta Bambery Crismas // Himiya rastitel'nogo syr'ya. 2022. № 1. S. 153–160. DOI:https://doi.org/10.14258/jcprm.2022019544.
26. Matraszec-Gawron R., Chwil M., Terlecka P., Skoczylas M. Recent studies on anti-depressant bioactive substances in selected species from genera Hemerocallis and Gladiolus: A systematic review // Psychology, Medicine, Pharmaceuticals. 2019. Vol. 12, No. 4. Article number 172. DOI:https://doi.org/10.3390/ph12040172.
27. Szewczyk K., Miazga-Karska M., Pierzak W., Komsta L., Krzeminska B., Grzywa-Celinska A. Phenolic cjmpositijn fnd skin-related properties of the aerial parts extract of different Hemerocallis cultivars // Medicine, Chemistry, Antioxidants. 2020. Vol. 9, No. 8. Article number 690. DOI:https://doi.org/10.3390/antiox9080690.
28. Bano A., Khan J. The effect of Pseuclomonas putida and Spermine of growth and bioactive metabolites of Hemerocallis fulva L. leaves // Russian Journal of Plant Physiology. 2022. Vol. 69. Article number 132. DOI:https://doi.org/10.1134/S1021443722060024.
29. Zhou X., Zhou S., Wei J., Zhou Y. Volatile metabolomies and chemometric study provide insight the formation of the characteristic cultivar aroma of Hemerocallis // Medicine. Food Chemistry. 2022. No. 404. Article number 134495. DOI: 101016/j.foodchem.2022.134495.
30. Li X. K., Cui J. L., Wang J. H., Wang M. L. Metabolic and transcriptional regulatory mechanisms of differential carbohydrates formation from flower buds to flowers of Hemerocallis citrina // Scientia Horticulturae. 2023. Vol. 308. Article number 111553. DOI:https://doi.org/10.1016/j.scienta.2022.111553.
31. Pincekova L., Janciova E., Berkes D., Gyepes R., Kalarovic A., Caletkova O. Total synthesis of Hemerocallisamine I paved by Gram-Scale synthesis of (2S, 4S0-4-Hydroxylutamic acid lactone // Molecules. 2023. Vol. 28, No. 5. Article number 2177. DOI:https://doi.org/10.3390/molecules28052177.
