Russian Federation
Russian Federation
Abstract. The purpose is the determination of the degree of influence of small absorbed doses of ionizing radiation on biochemical and hematological parameters during irradiation of peripheral blood samples of cattle in vitro. For the work, blood samples were taken from lactating cows kept under the influence of the background absorbed dose in the Krasnoyarsk Territory, followed by in vitro irradiation at doses of 5, 50, 500 mGy. Methods. Peripheral blood was taken from blood vessels from the tail vein into vacuum tubes with a coagulation activator, in vitro irradiation of samples was carried out on an installation equipped with a Cs-137 source, biochemical studies of blood serum were carried out using a PE-5400UF spectrophotometer. Hematological blood parameters were determined according to generally accepted methods. Results. Ionizing radiation in vitro in doses of 5 mGy, 50 mGy and 500 mGy of peripheral blood samples of cattle affects hematological parameters in different directions, which is characterized by wave-like fluctuations in hemoglobin values, a decrease in the content of erythrocytes and a decrease in the erythrocyte sedimentation rate. It was found that a single irradiation of peripheral blood samples of cows at a dose of 5 mGy reduced the concentration of beta-globulins and creatinine and did not affect the content of total protein and protein fractions. When exposed to ionizing radiation at a dose of 50 mGy, the content of total protein, the level of beta-globulins, creatinine decreased in blood samples, and the content of albumins and alpha-, gamma-globulins did not change. When irradiated at a dose of 500 mGy, the relative content of alpha-globulins, the concentration of ALT and AST decreased, the content of creatinine, total protein, albumins and beta-, gamma-globulins did not change. The stability of alkaline phosphatase, albumins and gamma globulins to ionizing effects at doses of 5 mGy, 50 mGy and 500 mGy has been established. Scientific novelty. The linear dependence of the concentration of alpha-globulins, AST and ALT on the absorbed dose was revealed, the numerical values of the approximation coefficients describing the decrease in concentration and biochemical parameters were determined. The inverse direct linear relationship between creatinine stability and absorbed dose has been established. Practical significance is the established digital values of the approximation coefficients of alpha globulins, AST and ALT can be used in the reconstruction of radiation dose values in the range of 5–50 mGy.
ionizing radiation, small doses, absorbed dose, blood, hematological, biochemical parameters
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