Abstract. In the course of the study, an updated database of the content of biologically active substances in aqueous extracts of propolis prepared at different temperatures and in different ways is presented. The purpose of the work was to select the optimal modes of preparation of aqueous propolis extracts, to determine the content of biologically active substances in freshly prepared propolis extracts and during storage. The studies were carried out in the laboratory of the Federal State Budgetary Scientific Institution “FNC of beekeeping”. The relevance of the work lies in the development of methods for extracting aqueous extracts of propolis at different temperatures (t = 20 °C, t = 93 °C), the use of ultrasound. Scientific novelty for the first time biologically active substances were determined by the method of determining antioxidant activity. Methods. The yield of extractives (mass fraction of solids) was determined by drying to constant weight according to GOST 28886-90. Determination of unsaturated compounds was performed according to GOST 28886-90. The determination of the hydrogen index (pH) was performed according to the Pharmacopoeia (1987) in our modification GOST 28886-90. Determination of flavonoid and other phenolic compounds was performed by photometry, according to GOST 28886-90 (total flavonoid compounds). Determination of flavan, flavanones, flavonols was carried out according to the method presented in the “Guidelines for methods of quality control and safety of biologically active food supplements” R 4.1.1672-0, 2004 in terms of routine. The antioxidant activity of propolis aqueous extracts was assessed by a method based on the reaction of KMnO4 in the presence of 0.24 M H2SO4 with the studied propolis solution, followed by conversion to quercetin in 1 ml or 1 g of the drug (patent 2170930). Results. The amount of solids during storage in the aqueous extract of propolis (method 1) decreased from 0.23 to 0.15 %; in an aqueous extract prepared using ultrasound (method 3) increased from 0.21 % to 0.23 %. The amount of solids in propolis aqueous extracts prepared by methods 2 and 4 decreased during storage from 0.55 to 0.49 % (method 2) and from 0.6 to 0.59 % (method 4).
propolis, water extract of propolis, flavonoid compounds, dry matter, pH value, antioxidant activity
1. Patent Rossii № RU2170930 C1 Maksimova T. V. [i dr.] []// Sposob opredeleniya antiokislitel'noy aktivnosti. [Elektronnyy resurs]. URL: https://elibrary.ru/item.asp?id=37868227 (data obrascheniya: 11.02.2022).
2. GOST 56104-2014. Produkty pischevye organicheskie. Terminy i opredeleniya [Elektronnyy resurs]. URL: https://docs.cntd.ru/document/1200113488 (data obrascheniya: 11.12.2021).
3. GOST 33980-2016. Produkciya organicheskogo proizvodstva. Pravila proizvodstva, pererabotki, markirovki i realizacii [Elektronnyy resurs]. URL: https://docs.cntd.ru/document/1200141713 (data obrascheniya: 11.12.2021).
4. Ozdal T., Ceylan F. D., Eroglu N., Kaplan M., Olgun E. O., Capanoglu E. Investigation of antioxidant capacity, bioaccessibility and LC-MS/MS phenolic profile of Turkish propolis // Food Research International. 2019. T. 122. Pp. 528-536. DOI:https://doi.org/10.1016/j.foodres.2019.05.028.
5. Castro C., Mura F., Valenzuela G., Figueroa C., Salinas R. M., Josep C. Z., Torres L., Fuguet E., Delporte S. Identification of phenolic compounds by HPLC-ESI-MS/MS and antioxidant activity from Chilean propolis // Food Research International. 2014. T. 64. Rp. 873-879. DOI:https://doi.org/10.1016/j.foodres.2014.08.050.
6. Monroy Y. M. Rodney Rodrigues A. F., Marili Rodrigue V. N., Sant’Ana A. S., Beatriz B. S, Cabral F. A. Brazilian green propolis extracts obtained by conventional processes and by processes at high pressure with supercritical carbon dioxide, ethanol and water // The Journal of Supercritical Fluids. 2017. T. 130. Rp. 189-197. DOI:https://doi.org/10.1016/j.supflu.2017.08.006.
7. Asem N., Abdul G. N. A., Abd Hapit N. H., Omar E. A. Correlation between total phenolic and flavonoid contents with antioxidant activity of Malaysian stingless bee propolis extract // Journal of Apicultural Research. 2020. No. 59. Pp. 437-442. DOI:https://doi.org/10.1080/00218839.2019.1684050.
8. Bankova V., Bertelli D., Borba R. et al. Standard methods for Apis mellifera propolis research [e-resource] // Journal of Apicultural Research. 2019. T. 58. No. 2. URL: https://www.tandfonline.com/doi/full/10.1080/00218839.2016.1222661 (date of reference: 04.02.2022).
9. Khan S. A., Aslam R., Makroo H. A. High pressure extraction and its application in the extraction of bio-active compounds: A review [e-resource] // Journal of Food Process Engineering. 2019. T. 42. No. 1. Article number e12896. URL: https://onlinelibrary.wiley.com/doi/abs/10.1111/jfpe.12896 (date of reference: 04.02.2022).
10. Taddeo V. A., Epifano F., Fiorito S., Genovese S. Comparison of different extraction methods and HPLC quantification of prenylated and unprenylated phenylpropanoids in raw Italian propolis [e-resource] // Journal of Pharmaceutical and Biomedical Analysis 2016. T. 129. Rp. 219-223. DOI:https://doi.org/10.1016/j.jpba.2016.07.006.
11. Oroian M., Ursachi F., Dranca F. Influence of ultrasonic amplitude, temperature, time and solvent concentration on bioactive compounds extraction from propolis [e-resource] // Ultrasonics Sonochemistry. 2020. T. 64. Article number 105021. URL: https://www.sciencedirect.com/science/article/abs/pii/S1350417719318656 (date of reference: 07.02.2022).
12. Devequi-Nunes D., Machado B. A. S., de Abreu Barreto G., Silva J. R., da Silva D. F., da Rocha J. L. C., Brandão H. N., Borges V. M., Umsza-Guez M. A. Chemical characterization and biological activity of six different extracts of propolis through conventional methods and supercritical extraction [e-resource] // PloS One. 2018. T. 13 (12). Article number e0207676. URL: https://journals.plos.org/plosone/article?idhttps://doi.org/10.1371/journal.pone.0207676 (date of reference: 08.02.2022).
13. Pavlova L. V., Platonov I. A., Novikova E. A., Pudovkina S. A. Ekstrakciya biologicheski aktivnyh soedineniy propolisa vodoy v subkriticheskih usloviyah // Novye dostizheniya v himii i himicheskoy tehnologii rastitel'nogo syr'ya: materialy VIII Vserossiyskoy konferencii. Barnaul, 2020. S. 88-89.
14. Bachevski D., Damevska K., Simeonovski V., Dimova M. Back to the basics: Propolis and COVID-19 [e-resource] // Dermatologic Therapy. 2020. T. 33 (4). Pp. 3-5. Article number e13780. URL: https://onlinelibrary.wiley.com/doi/full/10.1111/dth.13780 (date of reference: 10.02.2022).
15. Berretta A. A., Silveira D. M. A, Cóndor Capcha J. M., De Jong D. Propolis and its potential against SARS-CoV-2 infection mechanisms and COVID-19 disease [e-resource] // Biomed. Pharm. 2020. T. 131. Article number 110622. URL: https://www.sciencedirect.com/science/article/pii/S0753332220308155 (date of reference: 02.02.2022).
16. Kowacz M., Pollack G. H. Propolis-induced exclusion of colloids: Possible new mechanism of biological action [e-resource] // Colloid and interface science communications. 2020. T. 38. Article number 100307. URL: https://www.sciencedirect.com/science/article/pii/S221503822030087X (date of reference: 07.02.2022).
17. Anjum S. I. Ullah A., Khan K. A., Attaullah M., Khan H., Ali H., Dash C. K. Composition and functional properties of propolis (bee glue) // Saudi Jornal of Biological Sciences 2019: a review. T. 26. Pp. 1695-1703. URL: DOI:https://doi.org/10.1016/j.sjbs.2018.08.013.
18. Sforcin J. M. Biological properties and therapeutic applications of propolis. [e-resource] // Phytherapy Research. 2016. T. 30. Rp. 894-905.
19. Nichitoi M. M., Josceanu A. M., Isopescu R. D., Isopencu G. O., Geana E. I., Ciucure C. T., Lavric V. Polyphenolics profile effects upon the antioxidant and antimicrobial activity of propolis extracts [e-resource] // Scientific Reports. 2021. No. 11. Article number 20113. URL: https://pubmed.ncbi.nlm.nih.gov/34635677 (date of reference: 15.02.2022).
20. De Oliveira Reis J. H., de Abreu Barreto G., Cerqueira J. C., dos Anjos J. P., Andrade L. N., Padilha F. F., Druzian J. I., Machado B. A. S. Evaluation of the antioxidant profile and cytotoxic activity of red propolis extracts from different regions of northeastern Brazil obtained by conventional and ultrasound-assisted extraction [e-resource] // PloS One. 2019. T. 14 (7). Article number e0219063. URL: https://journals.plos.org/plosone/article?idhttps://doi.org/10.1371/journal.pone.0219063 (date of reference: 12.02.2022).
21. Yashin A. Ya., Vedenin A. N., Yashin Ya. I., Vasilevich N. I. Antivirusnye polifenoly-antioksidanty: struktura, pischevye istochniki i mehanizm deystviya // Laboratoriya i proizvodstvo. 2020. № 5. S. 76-86.
22. Korochkina P. S., Vasil'cova I. V. Antioksidantnyy status propolisa i pchelinogo podmora // Aktual'nye problemy agropromyshlennogo kompleksa: sbornik trudov nauchno-prakticheskoy konferencii prepodavateley, studentov, magistrantov i aspirantov, posvyaschennyy 80-letiyu Novosibirskogo GAU. Novosibirsk, 2016. S. 348-351.
23. Filippov I. N. Apiterapiya protiv koronavirusnoy infekcii COVID-19 // Sovremennye problemy pchelovodstva i apiterapii: materialy Mezhdunarodnoy nauchno-prakticheskoy konferencii. Rybnoe, 2021. S. 471-473.
24. Popravko S. A. Primenenie produktov pchelovodstva v apiterapii // Sovremennye problemy pchelovodstva i apiterapii: materialy Mezhdunarodnoy nauchno-prakticheskoy konferencii. Rybnoe, 2021. S. 432-437.
25. Kolesnikov V. A. Antioksidantnaya aktivnost' produktov pchelovodstva v sravnenii s sinteticheskimi antioksidantami pri eksperimental'noy katarakte // Apiterapiya segodnya: materialy XVIII Vserossiyskoy nauchnoy konferencii «Uspehi apiterapii». Rybnoe, 2016. S. 14-17.
26. Kolesnikova A. V., Kolesnikov O. Yu. Itogi issledovaniya effektivnosti apiproduktov v oftal'mologii // Sbornik nauchno-issledovatel'skih rabot po pchelovodstvu i apiterapii: sbornik trudov konferencii «Sostoyanie i perspektivy razvitiya sovremennogo pchelovodstva i apiterapii». Rybnoe, 2018. S. 154-158.
27. Skuratovskaya I. V., Yakovlev D. A., Lantushenko A. O. Vozdeystvie vodnogo ekstrakta propolisa na kletki bukkal'nogo epiteliya cheloveka pri vospalitel'nyh zabolevaniyah rotovoy polosti // Aktual'nye voprosy biologicheskoy fiziki i himii. BFFH-2017: materialy XII mezhdunarodnoy nauchno-tehnicheskoy konferencii. Sevastopol', 2017. S. 51-54.
28. Ivaschenko M. N., Kuimov I. A., Guschin V. A. Vliyanie propolisa na yadernye struktury kletok bukkal'nogo epiteliya pri kurenii // Apisfera: nauchnye dostizheniya v pchelovodstve i apiterapii: sbornik statey I Vserossiyskoy nauchno-prakticheskoy konferencii. Nizhniy Novgorod, 2019. S. 40-42.
29. Zulhendri F., Chandrasekaran K., Kowacz M., Ravalia M., Kripal K., Fearnley J., Perera C. O. Antiviral, Antibacterial, Antifungal, and Antiparasitic Properties of Propolis: A Review [e-resource] // Foods. 2021. T. 10 (6). Article number 1360. URL: https://www.mdpi.com/2304-8158/10/6/1360 (date of reference: 12.02.2022).
